IRS makes different measurements of the same thing all exactly the same and puts
all of the intensities on the same scale. Raw data should be taken as input (on_raw = TRUE)
Usage
irsNorm(se, ain = "raw", aout = "IRS", on_raw = TRUE)
Arguments
- se
SummarizedExperiment containing all necessary information of the proteomic dataset
- ain
String which assay should be used as input
- aout
String which assay should be used to save normalized data
- on_raw
Boolean specifying whether normalization should be performed on raw or log2-scaled data
Value
SummarizedExperiment containing the IRS normalized data as assay (on log2 scale)
Examples
data(tuberculosis_TMT_se)
tuberculosis_TMT_se <- irsNorm(tuberculosis_TMT_se, ain = "raw",
aout = "IRS", on_raw = TRUE)