Forces distributions of the samples to be the same on the basis of the quantiles of the samples by replacing
each protein of a sample with the mean of the corresponding quantile. Log2-scaled data should be taken as input (on_raw = FALSE)
Usage
quantileNorm(se, ain = "log2", aout = "Quantile", on_raw = FALSE)
Arguments
- se
SummarizedExperiment containing all necessary information of the proteomic dataset
- ain
String which assay should be used as input
- aout
String which assay should be used to save normalized data
- on_raw
Boolean specifying whether normalization should be performed on raw or log2-scaled data
Value
SummarizedExperiment containing the quantile normalized data as assay (on log2 scale)
Examples
data(tuberculosis_TMT_se)
tuberculosis_TMT_se <- quantileNorm(tuberculosis_TMT_se, ain = "log2",
aout = "Quantile", on_raw = FALSE)